Russell Biotech’s first product entry, Goat-anti-mouse-FMP (GAM-FMP) will permit rapid isolation/enrichment of cells of interest in high yield using negative selection. The use of negative selection yields cells that are free of bound magnetic particles and, thus, immediately available and untouched for research studies. GAM-FMP will allow the researcher to use antibodies of choice to remove the desired cell populations.
| Product | Product Number | Price |
| GAM-FMP, 1ml | C00001 | Inquire |
| GAM-FMP, 2ml | C00002 | Inquire |
Custom Particles: Russell Biotech will provide custom particles upon
request. Also, please inquire about our new avidin and anti-FITC particles.
Future product releases will include:
 | CD15-FMP for the rapid removal of CD15 positive cells | |
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CD41-FMP for the rapid removal of platelets, from undiluted whole blood, prior to Ficoll isolation of mononuclear cells: The pre-step yields very clean mononuclear preparations devoid of “sticky” platelets. |
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| T Cell Negative Isolation Kit that will yield highly enriched T cells | |
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| Monocyte Negative Isolation Kit that will yield highly enriched monocytes | . |
Equipment required for optimal performance of Quick-Sep FMP:
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Mixer: Proper
mixing is essential to ensure contact between the Ferromagnetic Particles and
the targeted cells.
For reaction volumes </= to 0.5ml mixing can be accomplished by vortexing at a low speed using a Vortex Genie 2 that has variable speed control. A Vortex Genie 2 with a timer is desirable but a timer is not essential. For volumes >/= 0.5ml up to 50ml mixing can be accomplished
by end-over-end mixing using an ATR Rotamix mixer (www.atrbiotech.com/benchtop/rotamix.htm)
with variable speed. Recommended mixing speed is 15 rpm. |
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Magnetic Separation: Ideal magnets that can be used with Quick-Sep Ferromagnetic
Particles can be obtained from Dexter Magnetic Technologies (www.lifesep.com
under products). Different magnets are available for sample volumes from </=
0.5ml to 50ml.
Magnets from suppliers of superparamagnetic particles will
also work with Quick-Sep Ferromagnetic Particles. |
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Demagnetizer: One of the unique positive attributes of
Russell Biotech’s particles is that the particles are ferromagnetic not
superparamagnetic. As a result the particles will maintain some magnetism when
the magnetic field is removed. Simple vortexing is able to overcome this
magnetic effect and captured cells are easily dispersed. However, especially
when the targeted cells are rare and/or the antigen density is relatively low,
the most efficient capture occurs with well dispersed particles. To ensure well
dispersed particles, it is recommended that the particles be demagnetized (30
second procedure) immediately prior to addition to the sample using a
demagnetizer/degausser from Data-Link Associates (www.datalinksales.com ); product number PF-211. |